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Addgene

pCS2FLAG
(Plasmid #16331)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16331 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4095
  • Vector type
    Vertebrate expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Flag tag
  • Alt name
    pCS2+Flag
  • Insert Size (bp)
    63
  • Mutation
    Flag tag inserted into pCS2+ polylinker
  • Tag / Fusion Protein
    • Flag

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site claI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

2xFLAG tag cloned into pCS2 for use as N- or C-terminal fusion tag. In same reading frame as myc tags of pCS2MT.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2FLAG was a gift from Peter Klein (Addgene plasmid # 16331 ; http://n2t.net/addgene:16331 ; RRID:Addgene_16331)
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