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PurposeExpresses FlipGFP-based activity reporter of SARS-CoV-2 main protease Mpro and also mCherry
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 163078 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFlipGFP-based Mpro activity reporter and mCherry
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SpeciesH. sapiens (human)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2020.10.28.359042 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3 FlipGFP(Mpro) T2A mCherry was a gift from Xiaokun Shu (Addgene plasmid # 163078 ; http://n2t.net/addgene:163078 ; RRID:Addgene_163078) -
For your References section:
Ethacridine inhibits SARS-CoV-2 by inactivating viral particles. Li X, Lidsky P, Xiao Y, Wu CT, Garcia-Knight M, Yang J, Nakayama T, Nayak JV, Jackson PK, Andino R, Shu X. PLoS Pathog. 2021 Sep 3;17(9):e1009898. doi: 10.1371/journal.ppat.1009898. 10.1371/journal.ppat.1009898 PubMed 34478458