pGL2-50bp
(Plasmid
#16295)
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 16295 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepGL2 basic
-
Backbone manufacturerpromega
- Backbone size w/o insert (bp) 5598
-
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namep53 promoter fragment
-
SpeciesH. sapiens (human)
-
Insert Size (bp)50
-
Mutationfragment -38 to +12 relative to first transcription initiation site.
-
Entrez GeneTP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer na (Common Sequencing Primers)
Resource Information
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pGL2-50bp was a gift from Wafik El-Deiry (Addgene plasmid # 16295 ; http://n2t.net/addgene:16295 ; RRID:Addgene_16295) -
For your References section:
p73 or p53 directly regulates human p53 transcription to maintain cell cycle checkpoints. Wang S, El-Deiry WS. Cancer Res. 2006 Jul 15. 66(14):6982-9. 10.1158/0008-5472.CAN-06-0511 PubMed 16849542