pHB9-EGFP (Hb9 promoter) [TJ#96]
(Plasmid #16275)

• Depositing Lab: Thomas Jessell
• Publication: Wilson et al J Neurosci. 2005 Jun 15. 25(24):5710-9.

Backbone

• Vector backbone: pBluescript
• Backbone size w/o insert (bp): 3000
• Vector type: Mammalian Expression, Mouse Targeting

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HB9 promoter
• Species: M. musculus (mouse)
• Insert Size (bp): 9000
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Approximately 9 kb fragment comprising the 5' upstream region of the murine HB9 gene, followed by a 5' splice substrate, an eGFP gene, and a bovine growth hormone polyadenylation signal.