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Addgene

8xNFAT-ZsG-mCD8
(Plasmid #162747)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162747 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSIR
  • Backbone manufacturer
    Maki Nakayama
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    8x of NFAT binding motif followed by ZsGreen-1 fluorescent protein gene
  • Species
    M. musculus (mouse)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Second gene is mouse CD8

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    8xNFAT-ZsG-mCD8 was a gift from Maki Nakayama (Addgene plasmid # 162747 ; http://n2t.net/addgene:162747 ; RRID:Addgene_162747)

  • For your References section:

    Multiplex T-cell Stimulation Assay Utilizing a T-cell Activation Reporter-based Detection System. Landry LG, Mann SE, Anderson AM, Nakayama M. Bio Protoc. 2021 Jan 20;11(2):e3883. doi: 10.21769/BioProtoc.3883. eCollection 2021 Jan 20. 10.21769/BioProtoc.3883 PubMed 33732772
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