ST-GFP
(Plasmid
#162500)
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PurposeExpresses ST6GAL1 with a GFP tag in its C-terminus in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 162500 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepmoxGFP-N1
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Backbone manufacturerA gift from E. Snapp (Addgene plasmid # 68070)
- Backbone size w/o insert (bp) 4697
- Total vector size (bp) 5918
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameST6Gal1
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SpeciesH. sapiens (human)
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Insert Size (bp)1221
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GenBank IDBC040009.1
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Entrez GeneST6GAL1 (a.k.a. CDw75, SIAT1, ST6GalI, ST6N)
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Tag
/ Fusion Protein
- GFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ST-GFP was a gift from Lei Lu (Addgene plasmid # 162500 ; http://n2t.net/addgene:162500 ; RRID:Addgene_162500) -
For your References section:
Glycans function as a Golgi export signal to promote the constitutive exocytic trafficking. Sun X, Tie HC, Chen B, Lu L. J Biol Chem. 2020 Aug 21. pii: RA120.014476. doi: 10.1074/jbc.RA120.014476. 10.1074/jbc.RA120.014476 PubMed 32826314
