pPEG
(Plasmid #162466)

• Purpose: (Empty Backbone) Entry vector for gRNA insertion in plant prime editing expression vector
• Depositing Lab: Adam Bogdanove
• Publication: Wang et al Front Genome Ed. 2021 Mar 4;3:617553. doi: 10.3389/fgeed.2021.617553. eCollection 2021.

Backbone

• Vector backbone: pCR8
• Backbone manufacturer: ThermoFisher
• Backbone size (bp): 2817
• Vector type: entry vector for gRNA
• Promoter: CmYLCV

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: M13 forward
• 3′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • pPEG.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

promoter and other sequence data from Cermak 2017 paper.
A Multipurpose Toolkit to Enable Advanced Genome Engineering in Plants. Cermak, T.
Curtin, S. J., Gil-Humanes, J., Cegan, R., Kono, T. J. Y., Konecna, E., Belanto, J. J., Starker, C. G., Mathre, J. W., Greenstein, R. L., Voytas, D. F. Plant Cell. 2017; 29(6):1196-1217.
Please visit https://www.biorxiv.org/content/10.1101/2020.07.16.206276v1 for bioRxiv preprint.