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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 16214 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameKCNT1 potassium channel, subfamily
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Alt namehSlo2.2
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Alt nameGI:73611943
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SpeciesH. sapiens (human)
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Insert Size (bp)4600
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MutationThis construct lacks the first 53aa when compared to GenBank reference sequence
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GenBank IDNM_020822
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Entrez GeneKCNT1 (a.k.a. DEE14, EIEE14, ENFL5, KCa4.1, SLACK, Slo2.2, bA100C15.2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Nhe1 (not destroyed)
- 3′ cloning site Xho1 (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that this plasmid does not contain the full-length hSlo2.2 (KCNT1) sequence. When compared to GenBank reference sequence NM_020822, the hSlo2.2 insert in this plasmid is missing the first 159 nucleotides of the coding region, which represent the first 53 amino acids of the hSlo2.2 protein sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3+ hSlo2.2 was a gift from Larry Salkoff (Addgene plasmid # 16214 ; http://n2t.net/addgene:16214 ; RRID:Addgene_16214)