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Addgene

pKAR2-Br512
(Plasmid #161875)

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Item Catalog # Description Quantity Price (USD)
Plasmid 161875 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pKAR2
  • Backbone manufacturer
    In-house made
  • Backbone size w/o insert (bp) 5187
  • Total vector size (bp) 5187
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Modified DNA Polymerase I Large Fragment (Br512)
  • Alt name
    Bst-LF
  • Species
    Geobacillus stearothermophilus
  • Insert Size (bp)
    1932
  • GenBank ID
  • Promoter T7, tetPA
  • Tag / Fusion Protein
    • 8x His tag; Villin Headpiece 47 amino acid (HP47) ; Linker

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)
  • 5′ sequencing primer GCATCGTCTCATCGGTCTCATATGCACCATCATCACGGTCATC
  • 3′ sequencing primer ATGCCGTCTCAGGTCTCAGGATCCTTATTACTTGGCGTCATACCAGGTG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKAR2-Br512 was a gift from Andrew Ellington (Addgene plasmid # 161875 ; http://n2t.net/addgene:161875 ; RRID:Addgene_161875)

  • For your References section:

    An improved and readily available version of Bst DNA Polymerase for LAMP, and applications to COVID-19 diagnostics. Maranhao A,Bhadra S, Paik I, Walker D, Ellington AD. medRxiv 10.1101/2020.10.02.20203356
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