pBSc + MShal
(Plasmid
#16166)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16166 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBSc
- Backbone size w/o insert (bp) 3200
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Vector typeXenopus Oocyte expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMus musculus potassium voltage-gated channel
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Alt nameGI:199812
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Alt nameM64226
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Alt nameMGI:96671
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SpeciesM. musculus (mouse)
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Insert Size (bp)3000
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MutationN/A
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GenBank IDNM_008423
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Entrez GeneKcnd1 (a.k.a. Shal, Kv4.1, Kca2-1, mShal1, 1110037K09Rik)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (not destroyed)
- 3′ cloning site EcoR1 (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBSc + MShal was a gift from Larry Salkoff (Addgene plasmid # 16166 ; http://n2t.net/addgene:16166 ; RRID:Addgene_16166) -
For your References section:
mShal, a subfamily of A-type K+ channel cloned from mammalian brain. Pak MD, Baker K, Covarrubias M, Butler A, Ratcliffe A, Salkoff L. Proc Natl Acad Sci U S A. 1991 May 15. 88(10):4386-90. 10.1073/pnas.88.10.4386 PubMed 2034678
Map uploaded by the depositor.
