Pmec-18 caspase-3 (p12)::nz [TU#813]
(Plasmid #16082)

• Depositing Lab: Martin Chalfie
• Publication: Chelur et al Proc Natl Acad Sci U S A. 2007 Feb 13. 104(7):2283-8.

Backbone

• Vector backbone: pPD95.75
• Backbone manufacturer: Andrew Fire Lab
• Backbone size w/o insert (bp): 3880
• Vector type: Worm Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: caspase-3 (p12) :: nz
• Alt name: caspase-3
• Species: H. sapiens (human)
• Insert Size (bp): 519
• Entrez Gene: CASP3 (a.k.a. CPP32, CPP32B, SCA-1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: M13 rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: caspase-3 from MGC full length cDNA clone ID 4419175.
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

caspase-3 (p12) fused to the antiparallel leucine-zipper domain NZ (ALKKELQANKKELAQLKWELQALKKELAQ). For listed sequence, 1-397bp = mec-18p; 410-718bp = caspase-3(p12); 746-787bp = intron; 824-916bp = NZ.