Phsp-16 cz::caspase-3 (p17) [TU#823]
(Plasmid
#16086)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 16086 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepPD49.78
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Backbone manufacturerAndrew Fire Lab
- Backbone size w/o insert (bp) 3914
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namecz :: caspase-3 (p17)
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Alt namecaspase-3
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SpeciesH. sapiens (human)
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Insert Size (bp)743
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Entrez GeneCASP3 (a.k.a. CPP32, CPP32B, SCA-1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI ? (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bycaspase-3 from MGC full length cDNA clone ID 4419175.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
caspase-3 (p17) fused to the antiparallel leucine-zipper domain CZ (AQLEKKLQALEKKLAQLEWKNQALEKKLAQ).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Phsp-16 cz::caspase-3 (p17) [TU#823] was a gift from Martin Chalfie (Addgene plasmid # 16086 ; http://n2t.net/addgene:16086 ; RRID:Addgene_16086) -
For your References section:
Targeted cell killing by reconstituted caspases. Chelur DS, Chalfie M. Proc Natl Acad Sci U S A. 2007 Feb 13. 104(7):2283-8. 10.1073/pnas.0610877104 PubMed 17283333