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Addgene

pKP1141
(Plasmid #160811)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160811 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPro33
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tonB
  • Species
    Escherichia coli
  • Entrez Gene
    tonB (a.k.a. b1252, ECK1246, T1rec, exbA)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Slient GCA-to-GCT mutation at TonB residue A165

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKP1141 was a gift from Kathleen Postle (Addgene plasmid # 160811 ; http://n2t.net/addgene:160811 ; RRID:Addgene_160811)

  • For your References section:

    Death of the TonB Shuttle Hypothesis. Gresock MG, Savenkova MI, Larsen RA, Ollis AA, Postle K. Front Microbiol. 2011 Oct 12;2:206. doi: 10.3389/fmicb.2011.00206. eCollection 2011. 10.3389/fmicb.2011.00206 PubMed 22016747
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