(194) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4,K852A)
(Plasmid #160742)

• Purpose: Expresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to the EPEA nanobody. A different nanobody can be inserted by using Sgfl + Sgsl restriction enzymes.
• Depositing Lab: Christina Woo
• Publication: Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-1066. doi: 10.1021/acschembio.0c00074. Epub 2020 Mar 2.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 8025
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HA-nEPEA-(EAAAK)4-OGT(4,K852A)
• Species: H. sapiens (human); llama glama
• Promoter: T7/CMV
• Tag / Fusion Protein:
  • HA-Tag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sgfl, BamHI (unknown if destroyed)
• 3′ cloning site: Sgsl, NotI (unknown if destroyed)
• 5′ sequencing primer: T7 fwd
• 3′ sequencing primer: BGH rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry