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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16057 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGL4.10 (luc2)
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 4200
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMultimerized E-box
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SpeciesM. musculus (mouse)
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Insert Size (bp)250
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Tag
/ Fusion Protein
- Luciferase (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV (unknown if destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer RVprimer3 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Contains three repeats of 5'-TTGGGAGGCAGCAGGTG-3'. p4RTK: has a minimal thymidine kinase promoter.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p4RTK GL4.10 (Luc2) was a gift from Robert Benezra (Addgene plasmid # 16057 ; http://n2t.net/addgene:16057 ; RRID:Addgene_16057) -
For your References section:
Transcription of the dominant-negative helix-loop-helix protein Id1 is regulated by a protein complex containing the immediate-early response gene Egr-1. Tournay O, Benezra R. Mol Cell Biol. 1996 May . 16(5):2418-30. PubMed 8628310