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Purpose(Empty Backbone) vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 160451 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFBDM
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Backbone manufacturerhttps://www.nature.com/articles/nbt1036
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Modifications to backboneWe chemically synthesized genetic elements derived from the previously described BacMam vector pVLAD (Dukkipati, A., Park, H.H., Waghray, D., Fischer, S. & Garcia, K.C. BacMam system for high-level expression of recombinant soluble and membrane glycoproteins for structural studies. Protein Expr. Purif. 62, 160–170 (2008)); a CMV promoter and synthetic intron for efficient RNA splicing and mRNA processing, and a WPRE motif for efficient mRNA processing, stability and export.
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Vector typeMammalian Expression
- Promoter CMV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEG BacMam was a gift from Eric Gouaux (Addgene plasmid # 160451 ; http://n2t.net/addgene:160451 ; RRID:Addgene_160451) -
For your References section:
Screening and large-scale expression of membrane proteins in mammalian cells for structural studies. Goehring A, Lee CH, Wang KH, Michel JC, Claxton DP, Baconguis I, Althoff T, Fischer S, Garcia KC, Gouaux E. Nat Protoc. 2014 Nov;9(11):2574-85. doi: 10.1038/nprot.2014.173. Epub 2014 Oct 9. 10.1038/nprot.2014.173 PubMed 25299155
Map uploaded by the depositor.
