pUCIDT-attL1-Human ABeta-attR5
(Plasmid
#160436)
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PurposeEntry vector for cloning human Amyloid Beta(1-42) using 2-fragment gateway recombination
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 160436 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUCIDT
- Total vector size (bp) 3061
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHomo sapiens amyloid beta precursor protein
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SpeciesH. sapiens (human)
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Entrez GeneAPP (a.k.a. AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, alpha-sAPP, preA4)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 For
- 3′ sequencing primer M13 Rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUCIDT-attL1-Human ABeta-attR5 was a gift from Nicholas Tolwinski (Addgene plasmid # 160436 ; http://n2t.net/addgene:160436 ; RRID:Addgene_160436) -
For your References section:
Use of Optogenetic Amyloid-beta to Monitor Protein Aggregation in Drosophila melanogaster, Danio rerio and Caenorhabditis elegans. Kaur P, Kibat C, Teo E, Gruber J, Mathuru A, Tolwinski ANS. Bio Protoc. 2020 Dec 5;10(23):e3856. doi: 10.21769/BioProtoc.3856. eCollection 2020 Dec 5. 10.21769/BioProtoc.3856 PubMed 33659494
