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Addgene

pGAL-PDE1
(Plasmid #160388)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160388 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLND46
  • Backbone manufacturer
    Genetics. 2009 Sep; 183(1): 365–383.
  • Backbone size w/o insert (bp) 3825
  • Total vector size (bp) 6759
  • Vector type
    Yeast Expression
  • Selectable markers
    Nourseothricin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PDE1
  • Alt name
    YGL248W
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    3110
  • Promoter GAL

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (destroyed during cloning)
  • 3′ cloning site SpeI (destroyed during cloning)
  • 5′ sequencing primer NA
  • 3′ sequencing primer NA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note plasmid contains the following mutations T114A, K153E, I201T and N306S in PDE1. Depositor confirms plasmid is functional.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGAL-PDE1 was a gift from John McCusker (Addgene plasmid # 160388 ; http://n2t.net/addgene:160388 ; RRID:Addgene_160388)
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