pCMV6 Myc PAK5 S573N
(Plasmid
#16021)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16021 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepCMV6-myc
- Backbone size w/o insert (bp) 4900
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namePAK5
-
SpeciesH. sapiens (human)
-
Insert Size (bp)2200
-
MutationS573N: constitutively active
-
Entrez GenePAK5 (a.k.a. PAK7)
-
Tag
/ Fusion Protein
- Myc (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI/BglII (destroyed during cloning)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Order of elements: SalI-Myc-XbaI-Pak5-EcoRI
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCMV6 Myc PAK5 S573N was a gift from Jonathan Chernoff (Addgene plasmid # 16021 ; http://n2t.net/addgene:16021 ; RRID:Addgene_16021) -
For your References section:
p21-Activated kinase 5 (Pak5) localizes to mitochondria and inhibits apoptosis by phosphorylating BAD. Cotteret S, Jaffer ZM, Beeser A, Chernoff J. Mol Cell Biol. 2003 Aug . 23(16):5526-39. 10.1128/MCB.23.16.5526-5539.2003 PubMed 12897128