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pLenti6.2_3xFLAG_V5_ mmDdx58_V5
(Plasmid #160108)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 160108 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti6.2 backbone
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ddx58
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Rigi (a.k.a. 6430573D20Rik, C330021E21, Ddx58, RIG-I, RLR-1)
  • Tag / Fusion Protein
    • 3xFLAG, V5 (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti6.2_3xFLAG_V5_ mmDdx58_V5 was a gift from Rizwan Haq (Addgene plasmid # 160108 ; http://n2t.net/addgene:160108 ; RRID:Addgene_160108)

  • For your References section:

    Inactivation of Fbxw7 impairs dsRNA sensing and confers resistance to PD-1 blockade. Gstalder C, Liu D, Miao D, Lutterbach B, DeVine AL, Lin C, Shettigar M, Pancholi P, Buchbinder EI, Carter SL, Manos MP, Rojas-Rudilla V, Brennick R, Gjini E, Chen PH, Lako A, Rodig S, Yoon CH, Freeman GJ, Barbie DA, Hodi FS, Miles W, Van Allen EM, Haq R. Cancer Discov. 2020 May 5. pii: 2159-8290.CD-19-1416. doi: 10.1158/2159-8290.CD-19-1416. 10.1158/2159-8290.CD-19-1416 PubMed 32371478
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