pPB-CAG-Brainbow3.2-PGK-PuroTK
(Plasmid #159928)

• Purpose: Brainbow transgenesis
• Depositing Lab: Hideyuki Okano
• Publication: Yoshimatsu et al Stem Cell Res. 2021 Jan 8;51:102164. doi: 10.1016/j.scr.2021.102164.

Backbone

• Vector backbone: pPB-MCSfwd
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: NFPnls
• Species: Synthetic
• Insert Size (bp): 705

Gene/Insert 2

• Gene/Insert name: mOrange2
• Species: Synthetic
• Insert Size (bp): 708

Gene/Insert 3

• Gene/Insert name: EGFP
• Species: Synthetic
• Insert Size (bp): 717

Gene/Insert 4

• Gene/Insert name: mKate2
• Species: Synthetic
• Insert Size (bp): 696

Resource Information

• Supplemental Documents:
  • pPB-CAG-Brainbow3.2-pPuroTK.gb
• A portion of this plasmid was derived from a plasmid made by: Joshua Sanes

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
  • piggyBac Limited Use Label License
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Brainbow3.2 cassette was derived from pThy1-Brainbow3.2 (https://www.addgene.org/45179/)

Addgene QC identified several ambiguous bases in the backbone sequence that could not be resolved by standard NGS. These ambiguities are present in the backbone sequence and are not of concern for the function of the construct.