R701-X65-527: His6-tev-Hs.CALM1(2-148)
(Plasmid
#159693)
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PurposeE. coli protein expression of His6-tev-Hs.CALM1(2-148)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 159693 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDest-527
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameHis6-tev-Hs.CALM1(2-148)
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SpeciesH. sapiens (human)
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Entrez GeneCALM1 (a.k.a. CALML2, CAM2, CAM3, CAMB, CAMC, CAMI, CAMIII, CPVT4, DD132, LQT14, PHKD, PHKD1, caM)
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Tag
/ Fusion Protein
- His6-tev (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
R701-X65-527: His6-tev-Hs.CALM1(2-148) was a gift from Dominic Esposito (Addgene plasmid # 159693 ; http://n2t.net/addgene:159693 ; RRID:Addgene_159693) -
For your References section:
KRAS Prenylation Is Required for Bivalent Binding with Calmodulin in a Nucleotide-Independent Manner. Agamasu C, Ghirlando R, Taylor T, Messing S, Tran TH, Bindu L, Tonelli M, Nissley DV, McCormick F, Stephen AG. Biophys J. 2019 Mar 19;116(6):1049-1063. doi: 10.1016/j.bpj.2019.02.004. Epub 2019 Feb 15. 10.1016/j.bpj.2019.02.004 PubMed 30846362