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Addgene

R701-X65-527: His6-tev-Hs.CALM1(2-148)
(Plasmid #159693)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159693 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pDest-527
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    His6-tev-Hs.CALM1(2-148)
  • Species
    H. sapiens (human)
  • Entrez Gene
    CALM1 (a.k.a. CALML2, CAM2, CAM3, CAMB, CAMC, CAMI, CAMIII, CPVT4, DD132, LQT14, PHKD, PHKD1, caM)
  • Tag / Fusion Protein
    • His6-tev (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    R701-X65-527: His6-tev-Hs.CALM1(2-148) was a gift from Dominic Esposito (Addgene plasmid # 159693 ; http://n2t.net/addgene:159693 ; RRID:Addgene_159693)
  • For your References section:

    KRAS Prenylation Is Required for Bivalent Binding with Calmodulin in a Nucleotide-Independent Manner. Agamasu C, Ghirlando R, Taylor T, Messing S, Tran TH, Bindu L, Tonelli M, Nissley DV, McCormick F, Stephen AG. Biophys J. 2019 Mar 19;116(6):1049-1063. doi: 10.1016/j.bpj.2019.02.004. Epub 2019 Feb 15. 10.1016/j.bpj.2019.02.004 PubMed 30846362