pLOVE-Klf4
(Plasmid #15950)

• Depositing Lab: Miguel Ramalho-Santos
• Publication: Blelloch et al Cell Stem Cell. 2007 Sep 13. ():.

Backbone

• Vector backbone: pLOVE
• Backbone size w/o insert (bp): 8263
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Expand pLOVE/GeneX in Stbl3 cells(sensitive to ccdB)
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Kruppel-like factor 4
• Alt name: Klf4, GKLF, EZF
• Species: M. musculus (mouse)
• Insert Size (bp): 2156
• Mutation: The recombination reaction required to clone "Gene X" into the pLOVE vector backbone removes 1456 bp (the CmR-ccdB region). Therefore, the size of pLOVE-Klf4 is ~8963 bp.
• Entrez Gene: Klf4 (a.k.a. EZF, Gklf, Zie)
• Tag / Fusion Protein:
  • V5 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a
• 3′ sequencing primer: V5

Resource Information

• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry