pLOVE-GFP
(Plasmid #15949)

• Depositing Lab: Miguel Ramalho-Santos
• Publication: Blelloch et al Cell Stem Cell. 2007 Sep 13. ():.

Backbone

• Vector backbone: pLOVE
• Backbone size w/o insert (bp): 8263
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Expand pLOVE/GeneX in Stbl3 cells(sensitive to ccdB)
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Green Fluorescent Protein
• Alt name: EGFP
• Species: Aequorea victoria
• Insert Size (bp): 600
• Mutation: The recombination reaction required to clone "Gene X" into the pLOVE vector backbone removes 1456 bp (the CmR-ccdB region). Therefore, the size of pLOVE-GFP is ~7407 bp.
• Tag / Fusion Protein:
  • V5 (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a
• 3′ sequencing primer: V5

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry