FRT LAMP3 mCRISPRed_mTagBFP2 pcDNA5
(Plasmid #159455)

• Purpose: Encodes a lysosome tagret fusion protein of the pH resistant proteins mCRISPRed and mTagBFP2
• Depositing Lab: Oliver Griesbeck
• Publication: Erdogan et al Cell Chem Biol. 2020 May 21;27(5):610-621.e5. doi: 10.1016/j.chembiol.2020.02.004. Epub 2020 Mar 5.

Backbone

• Vector backbone: pcDNA5/FRT
• Backbone manufacturer: Invitrogen/Thermofisher Scientific
• Backbone size w/o insert (bp): 5065
• Total vector size (bp): 7343
• Vector type: Mammalian Expression
• Selectable markers: Puromycin, Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCRISPRed
• Species: Entacmaea quadricolor
• Insert Size (bp): 708
• GenBank ID: MN844880.1
• Promoter: CMV
• Tag / Fusion Protein:
  • mTagBFP2 (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: TAATACGACTCACTATAGG
• 3′ sequencing primer: ATTTAGGTGACACTATAG

Resource Information

• Supplemental Documents:
  • FRT_LAMP3_mCRISPRed_mTagBFP2_pcDNA5.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry