pEF6-Lck-TurboID
(Plasmid
#159433)
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Purposeexpresses Lck-TurboID in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 159433 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEF6
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5818
- Total vector size (bp) 8249
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Vector typeMammalian Expression
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameLck
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Alt nameLck tyrosine kinase
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SpeciesH. sapiens (human)
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Insert Size (bp)1527
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GenBank IDNM_001042771.2
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Entrez GeneLCK (a.k.a. IMD22, LSK, YT16, p56lck, pp58lck)
- Promoter EF-1a
Cloning Information for Gene/Insert 1
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site BbvCI (unknown if destroyed)
- 5′ sequencing primer TATATGAATTCGCCATGGGCTGTGGCTGC
- 3′ sequencing primer ATATGTTTAAACTCAAGCGTAATCTGGAACATCGTATGGGTAAGGCTGAGGCTGGTACTG (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameTurboID (BirA mutant)
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SpeciesE. coli
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Insert Size (bp)969
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MutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V160A, T192A, K194I, M209V, M241T, S263P, I305V
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information for Gene/Insert 2
- Cloning method Restriction Enzyme
- 5′ cloning site BbvCI (unknown if destroyed)
- 3′ cloning site PmeI (unknown if destroyed)
- 5′ sequencing primer ATATGTTTAAACTCAAGCGTAATCTGGAACATCGTATGGGTAAGGCTGAGGCTGGTACTG
- 3′ sequencing primer TATATGTTTAAACTTAAGCGTAATCTGGAACATCGTATGGGTATCCGTCCAGGGTCAGGCGCTCC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byTurboID obtained from Addgene #107169 (Alice Ting Lab)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEF6-Lck-TurboID was a gift from Arthur Salomon (Addgene plasmid # 159433 ; http://n2t.net/addgene:159433 ; RRID:Addgene_159433) -
For your References section:
Quantitative Interactomics of Lck-TurboID in Living Human T Cells Unveils T Cell Receptor Stimulation-Induced Proximal Lck Interactors. Chua XY, Aballo T, Elnemer W, Tran M, Salomon A. J Proteome Res. 2020 Nov 13. doi: 10.1021/acs.jproteome.0c00616. 10.1021/acs.jproteome.0c00616 PubMed 33185455