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Addgene

pEF6-Lck-TurboID
(Plasmid #159433)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 159433 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEF6
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5818
  • Total vector size (bp) 8249
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Lck
  • Alt name
    Lck tyrosine kinase
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1527
  • GenBank ID
    NM_001042771.2
  • Entrez Gene
    LCK (a.k.a. IMD22, LSK, YT16, p56lck, pp58lck)
  • Promoter EF-1a

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site BbvCI (unknown if destroyed)
  • 5′ sequencing primer TATATGAATTCGCCATGGGCTGTGGCTGC
  • 3′ sequencing primer ATATGTTTAAACTCAAGCGTAATCTGGAACATCGTATGGGTAAGGCTGAGGCTGGTACTG
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    TurboID (BirA mutant)
  • Species
    E. coli
  • Insert Size (bp)
    969
  • Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V160A, T192A, K194I, M209V, M241T, S263P, I305V
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbvCI (unknown if destroyed)
  • 3′ cloning site PmeI (unknown if destroyed)
  • 5′ sequencing primer ATATGTTTAAACTCAAGCGTAATCTGGAACATCGTATGGGTAAGGCTGAGGCTGGTACTG
  • 3′ sequencing primer TATATGTTTAAACTTAAGCGTAATCTGGAACATCGTATGGGTATCCGTCCAGGGTCAGGCGCTCC
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    TurboID obtained from Addgene #107169 (Alice Ting Lab)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF6-Lck-TurboID was a gift from Arthur Salomon (Addgene plasmid # 159433 ; http://n2t.net/addgene:159433 ; RRID:Addgene_159433)
  • For your References section:

    Quantitative Interactomics of Lck-TurboID in Living Human T Cells Unveils T Cell Receptor Stimulation-Induced Proximal Lck Interactors. Chua XY, Aballo T, Elnemer W, Tran M, Salomon A. J Proteome Res. 2020 Nov 13. doi: 10.1021/acs.jproteome.0c00616. 10.1021/acs.jproteome.0c00616 PubMed 33185455