pKLV-EF1amCherry-2A-OsTIR1-W
(Plasmid #159296)

• Purpose: Lentiviral vector expressing mCherry and OsTIR1 driven by human EF1a promoter for the auxin-inducible degron (AID) system
• Depositing Lab: Kosuke Yusa
• Publication: Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi: 10.1038/s41375-020-1001-z. Epub 2020 Aug 6.

Backbone

• Vector backbone: pKLV-EF1amCherry
• Backbone size w/o insert (bp): 8811
• Total vector size (bp): 10539
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: OsTIR
• Insert Size (bp): 1728
• Promoter: human EF1a promoter

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: EF1a-seqU1

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2020.04.25.054049v1 for bioRxiv preprint.