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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15908 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRK5-myc
- Backbone size w/o insert (bp) 4750
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePOSH
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SpeciesM. musculus (mouse)
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Insert Size (bp)2700
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Entrez GeneSh3rf1 (a.k.a. 2200003J05Rik, P, Posh, R75531, Sh3md, Sh3md2)
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Tag
/ Fusion Protein
- myc (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRK5myc POSH was a gift from Alan Hall (Addgene plasmid # 15908 ; http://n2t.net/addgene:15908 ; RRID:Addgene_15908) -
For your References section:
A new rac target POSH is an SH3-containing scaffold protein involved in the JNK and NF-kappaB signalling pathways. Tapon N, Nagata K, Lamarche N, Hall A. EMBO J. 1998 Mar 2. 17(5):1395-404. 10.1093/emboj/17.5.1395 PubMed 9482736