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pTol2 hsp70l loxP-DsRed-loxP-nCas9n-GFP
(Plasmid #158962)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 158962 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript
  • Total vector size (bp) 13940
  • Vector type
    Cre/Lox, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    hsp70l promoter
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1443

Gene/Insert 2

  • Gene/Insert name
    floxed DsRed cassette
  • Species
    Synthetic
  • Insert Size (bp)
    1253

Gene/Insert 3

  • Gene/Insert name
    Cas9-GFP
  • Species
    Synthetic
  • Insert Size (bp)
    5134

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTol2 hsp70l loxP-DsRed-loxP-nCas9n-GFP was a gift from Michael Brand (Addgene plasmid # 158962 ; http://n2t.net/addgene:158962 ; RRID:Addgene_158962)

  • For your References section:

    Cre-Controlled CRISPR mutagenesis provides fast and easy conditional gene inactivation in zebrafish. Hans S, Zoller D, Hammer J, Stucke J, Spiess S, Kesavan G, Kroehne V, Eguiguren JS, Ezhkova D, Petzold A, Dahl A, Brand M. Nat Commun. 2021 Feb 18;12(1):1125. doi: 10.1038/s41467-021-21427-6. 10.1038/s41467-021-21427-6 PubMed 33602923
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