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5xUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2
(Plasmid #158651)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 158651 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    (na) pTol2mini?
  • Total vector size (bp) 8318
  • Vector type
    Tol2-based transgenic expression in zebrafish/other

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    5xUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y;he:BFP
  • Species
    Escherichia coli
  • Mutation
    F70A/F108Y

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    5xUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2 was a gift from Jeff Mumm (Addgene plasmid # 158651 ; http://n2t.net/addgene:158651 ; RRID:Addgene_158651)

  • For your References section:

    NTR 2.0: a rationally engineered prodrug-converting enzyme with substantially enhanced efficacy for targeted cell ablation. Sharrock AV, Mulligan TS, Hall KR, Williams EM, White DT, Zhang L, Emmerich K, Matthews F, Nimmagadda S, Washington S, Le KD, Meir-Levi D, Cox OL, Saxena MT, Calof AL, Lopez-Burks ME, Lander AD, Ding D, Ji H, Ackerley DF, Mumm JS. Nat Methods. 2022 Feb;19(2):205-215. doi: 10.1038/s41592-021-01364-4. Epub 2022 Feb 7. 10.1038/s41592-021-01364-4 PubMed 35132245
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