pLEX307-APN-G418
(Plasmid
#158456)
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PurposeConstitutive lentiviral expression of human APN.
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Depositing Labs
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 158456 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLEX307
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Vector typeMammalian Expression, Lentiviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAPN
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SpeciesH. sapiens (human)
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Entrez GeneANPEP (a.k.a. AP-M, AP-N, APN, CD13, GP150, LAP1, P150, PEPN, hAPN)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer EF1a-F
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLEX307-APN-G418 was a gift from Alejandro Chavez & Sho Iketani (Addgene plasmid # 158456 ; http://n2t.net/addgene:158456 ; RRID:Addgene_158456)
