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PurposeConstitutive lentiviral expression of human TMPRSS2.
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Depositing Labs
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 158459 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLEX307
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Vector typeMammalian Expression, Lentiviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTMPRSS2
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SpeciesH. sapiens (human)
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Entrez GeneTMPRSS2 (a.k.a. PRSS10)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer EF1a-F
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLEX307-TMPRSS2-G418 was a gift from Alejandro Chavez & Sho Iketani (Addgene plasmid # 158459 ; http://n2t.net/addgene:158459 ; RRID:Addgene_158459)