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Direct-seq_lentiGuide-Puro-tRNA-Loop2-8A8G
(Plasmid #157986)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 157986 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    lentiGuide-Puro (Plasmid#52963)
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin ; mkate2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    S. pyogenes sgRNA cassette (tRNA-Loop2-8A8G)
  • Alt name
    mkate2
  • gRNA/shRNA sequence
    unknown
  • Species
    Synthetic
  • Promoter hU6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (not destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer hU6-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Direct-seq_lentiGuide-Puro-tRNA-Loop2-8A8G was a gift from Lijia Ma (Addgene plasmid # 157986 ; http://n2t.net/addgene:157986 ; RRID:Addgene_157986)

  • For your References section:

    Direct-seq: programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen. Song Q, Ni K, Liu M, Li Y, Wang L, Wang Y, Liu Y, Yu Z, Qi Y, Lu Z, Ma L. Genome Biol. 2020 Jun 8;21(1):136. doi: 10.1186/s13059-020-02044-w. 10.1186/s13059-020-02044-w PubMed 32513233
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