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Addgene

pMAH-pnGFP-Ultra
(Plasmid #157924)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 157924 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMAH
  • Backbone size w/o insert (bp) 6981
  • Total vector size (bp) 7761
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pnGFP-Ultra
  • Insert Size (bp)
    780
  • Promoter cmv

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ACA CAT ACG ATT TAG GTG ACA CTA TAG AAT
  • 3′ sequencing primer ATGAAAGCCATACGGGAAGCAATAGC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMAH-pnGFP-Ultra was a gift from Huiwang Ai (Addgene plasmid # 157924 ; http://n2t.net/addgene:157924 ; RRID:Addgene_157924)

  • For your References section:

    A high-performance genetically encoded fluorescent biosensor for imaging physiological peroxynitrite. Chen Z, Zhang S, Li X, Ai HW. Cell Chem Biol. 2021 Jan 27. pii: S2451-9456(21)00013-1. doi: 10.1016/j.chembiol.2021.01.013. 10.1016/j.chembiol.2021.01.013 PubMed 33581056
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