pVL1392 TMEM97
(Plasmid
#157748)
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PurposeBaculovirus transfer vectors to create a virus expressing human TMEM97
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 157748 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepVL1392
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 9639
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTMEM97
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SpeciesH. sapiens (human)
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Insert Size (bp)558
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Entrez GeneTMEM97 (a.k.a. MAC30)
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Tag
/ Fusion Protein
- flag (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pVL1392 TMEM97 was a gift from Andrew Kruse (Addgene plasmid # 157748 ; http://n2t.net/addgene:157748 ; RRID:Addgene_157748) -
For your References section:
Identification of the gene that codes for the sigma2 receptor. Alon A, Schmidt HR, Wood MD, Sahn JJ, Martin SF, Kruse AC. Proc Natl Acad Sci U S A. 2017 Jul 3;114(27):7160-7165. doi: 10.1073/pnas.1705154114. Epub 2017 May 30. 10.1073/pnas.1705154114 PubMed 28559337
