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Addgene

pGSTag Flag Jnk3a2(2nd methione)
(Plasmid #15747)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15747 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGSTag
  • Backbone size w/o insert (bp) 5057
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Jnk3a2(2nd methione)
  • Alt name
    MAPK10
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1287
  • Entrez Gene
    MAPK10 (a.k.a. JNK3, JNK3A, PRKM10, SAPK1b, p493F12, p54bSAPK)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bam HI (not destroyed)
  • 3′ cloning site xho 1 (not destroyed)
  • 5′ sequencing primer pGex 5'
  • 3′ sequencing primer pGex 3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGSTag Flag Jnk3a2(2nd methione) was a gift from Roger Davis (Addgene plasmid # 15747 ; http://n2t.net/addgene:15747 ; RRID:Addgene_15747)

  • For your References section:

    Selective interaction of JNK protein kinase isoforms with transcription factors. Gupta S, Barrett T, Whitmarsh AJ, Cavanagh J, Sluss HK, Derijard B, Davis RJ. EMBO J. 1996 Jun 3. 15(11):2760-70. PubMed 8654373
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