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Addgene

pRetroSuper-GFP Smad3
(Plasmid #15723)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15723 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRetrosuper-GFP
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Mammalian Expression, Retroviral, RNAi
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Smad3 shRNA
  • Alt name
    Smad3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    450
  • Entrez Gene
    SMAD3 (a.k.a. HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3, hMAD-3, hSMAD3, mad3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer H1
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The following oligos were first cloned into pRetrosuper-Puro digested with BglII and HindIII.

Smad3-shRNA-sense: 5’-gatccccGGACGAGGTCTGCGTGAATttcaagagaATTCACGCAGACCTCGTCCtttttggaaa-3’

Smad3-shRNA-antisense: 5’-agcttttccaaaaaGGACGAGGTCTGCGTGAATtctcttgaaATTCACGCAGACCTCGTCCggg-3’

Next, the AgeI/XhoI fragments from shRNA sequence-containing pRetrosuper-Puro plasmids were cloned into the AgeI/XhoI site of pRetrosuper-GFP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRetroSuper-GFP Smad3 was a gift from Joan Massague (Addgene plasmid # 15723 ; http://n2t.net/addgene:15723 ; RRID:Addgene_15723)

  • For your References section:

    Hematopoiesis controlled by distinct TIF1gamma and Smad4 branches of the TGFbeta pathway. He W, Dorn DC, Erdjument-Bromage H, Tempst P, Moore MA, Massague J. Cell. 2006 Jun 2. 125(5):929-41. 10.1016/j.cell.2006.03.045 PubMed 16751102
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