pcDNA3 Flag hDPY30
(Plasmid #15554)

• Depositing Lab: Kai Ge
• Publication: Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-406.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5446
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DPY30
• Alt name: DPY-30
• Species: H. sapiens (human)
• Insert Size (bp): 400
• GenBank ID: NM_032574
• Entrez Gene: DPY30 (a.k.a. Cps25, HDPY-30, Saf19)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

FLAG-tagged hDPY-30 was cloned by PCR from HeLaS cDNA using primers 5'-C AGT AGG ATC CTG GCC ACC ATG GAC TAC AAG GAC GAC GAT GAC AAG ATG GAG CCA GAG CAG ATG CTG G-3' and 5'-TCG CTG CTC GAG TCA GTT TCG ATC TTC AAA CTG TGC-3'.