pcDNA3 Ash2L full
(Plasmid #15548)

• Depositing Lab: Kai Ge
• Publication: Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-406.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5446
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ash2L
• Species: H. sapiens (human)
• Insert Size (bp): 1800
• Mutation: R622H (Please see depositor comments below)
• GenBank ID: BC015936
• Entrez Gene: ASH2L (a.k.a. ASH2, ASH2L1, ASH2L2, Bre2)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Full-length ASH2L cDNA was amplified by reverse transcription-PCR from HeLaS cells using primers 5'-TAT AGG ATC CTG GTG ATG GCG GCG GCA GGA G-3' and 5'-CAG CGA CTC GAG TCA GGG TTC CCA TGG GGG AC-3'

Please note that mutation R622H in ASH2L was found during Addgene's quality control. The plasmid should function as described.