PET-3a-chpBK
(Plasmid
#155201)
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PurposeTo express recombinant ChpBK protein
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 155201 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePET-3a
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsFor protein expression, use BL21(DE3) E. coli
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert namechpS-chpBK-6×His-Tag
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SpeciesEscherichia coli
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Tag
/ Fusion Protein
- 6xHis
Cloning Information for Gene/Insert 1
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer - (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameGyrA-CBD-10xHis-Tag
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Tag
/ Fusion Protein
- 10xHis
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PET-3a-chpBK was a gift from Guanzheng Luo (Addgene plasmid # 155201 ; http://n2t.net/addgene:155201 ; RRID:Addgene_155201) -
For your References section:
Single-base mapping of m(6)A by an antibody-independent method. Zhang Z, Chen LQ, Zhao YL, Yang CG, Roundtree IA, Zhang Z, Ren J, Xie W, He C, Luo GZ. Sci Adv. 2019 Jul 3;5(7):eaax0250. doi: 10.1126/sciadv.aax0250. eCollection 2019 Jul. 10.1126/sciadv.aax0250 PubMed 31281898
