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Addgene

pLenti6.2_mCherry_ mito_roGFP2-Orp1
(Plasmid #155042)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 155042 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti6.2
  • Total vector size (bp) 10175
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Orp1
  • Alt name
    GPX3_YEAST
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    HYR1 (a.k.a. YIR037W, GPX3, ORP1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • roGFP2 (N terminal on insert)
    • mitochondrial targeting sequence (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer CMV-FW
  • 3′ sequencing primer WPRE-RV
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti6.2_mCherry_ mito_roGFP2-Orp1 was a gift from Vanessa LaPointe (Addgene plasmid # 155042 ; http://n2t.net/addgene:155042 ; RRID:Addgene_155042)
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