pDragon-empty
(Plasmid
#155018)
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Purpose(Empty Backbone) Empty Tigre-Dragon vector. Genes of interest can be cloned using Mlu-I and SnaB-I. For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 155018 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneAi62
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Backbone manufacturerAllen Institute
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Vector typeMouse Targeting
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Selectable markersSplit Hygro
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Based on Ai62 from Allen Institute
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDragon-empty was a gift from Alberto Rosello-Diez (Addgene plasmid # 155018 ; http://n2t.net/addgene:155018 ; RRID:Addgene_155018) -
For your References section:
A collection of genetic mouse lines and related tools for inducible and reversible intersectional mis-expression. Ahmadzadeh E, Bayin NS, Qu X, Singh A, Madisen L, Stephen D, Zeng H, Joyner AL, Rosello-Diez A. Development. 2020 May 28;147(10). pii: dev.186650. doi: 10.1242/dev.186650. 10.1242/dev.186650 PubMed 32366677
