rGSKmyc-R85-pXT7
(Plasmid
#15493)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15493 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepXT7-myc
- Backbone size w/o insert (bp) 3300
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Vector typeXenopus RNA expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGSK-3 beta R85
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Alt nameGSK-3 beta
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SpeciesR. norvegicus (rat)
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Insert Size (bp)1300
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MutationR85: K85R, dominant negative
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Entrez GeneGsk3b
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Tag
/ Fusion Protein
- myc (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (difficult due to 3' UTR) (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See author's map for details.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
rGSKmyc-R85-pXT7 was a gift from Sergei Sokol (Addgene plasmid # 15493 ; http://n2t.net/addgene:15493 ; RRID:Addgene_15493) -
For your References section:
Role of glycogen synthase kinase 3 beta as a negative regulator of dorsoventral axis formation in Xenopus embryos. Dominguez I, Itoh K, Sokol SY. Proc Natl Acad Sci U S A. 1995 Aug 29. 92(18):8498-502. 10.1073/pnas.92.18.8498 PubMed 7667318
Map uploaded by the depositor.
