pKM3
(Plasmid
#154168)
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Purpose(Empty Backbone) Empty vector with FLAG tag expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 154168 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepIEx
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Modifications to backboneIE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKM3 was a gift from Kevin Maringer (Addgene plasmid # 154168 ; http://n2t.net/addgene:154168 ; RRID:Addgene_154168) -
For your References section:
Imd pathway-specific immune assays reveal NF-kappaB stimulation by viral RNA PAMPs in Aedes aegypti Aag2 cells. Russell TA, Ayaz A, Davidson AD, Fernandez-Sesma A, Maringer K. PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0008524. doi: 10.1371/journal.pntd.0008524. 10.1371/journal.pntd.0008524 PubMed 33591970