Lenti-AcGFP-Sec61-IRES-Blast
(Plasmid #154009)

• Purpose: Lentiviral vector plasmid for integration and constitutive expression of AcGFP fused to Sec61 in mammalian cells – gift from D. Gerlich (IMBA, Vienna)
• Depositing Lab: Josef Penninger
• Publication: Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 10.1038/s42003-020-1072-4.

Backbone

• Vector backbone: pLV-CMV-EF1a-IRES-Blast
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: acGFP-Sec61
• Species: H. sapiens (human)
• Entrez Gene: SEC61B

Resource Information

• Supplemental Documents:
  • Lenti-AcGFP-Sec61-IRES-Blast.gb
• A portion of this plasmid was derived from a plasmid made by: This plasmid was generously provided by Matthias Samwer from the lab of Daniel Gerlich at the Institute of Molecular Biotechnology (IMBA) in Vienna Austria.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry