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PB-U6-DR-SgRNA-DR-EF1a-mCherry-SV40
(Plasmid #154002)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 154002 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PB
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SgRNA expression system
  • Species
    Synthetic
  • Insert Size (bp)
    1769
  • Promoter U6, EF1a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

INSERT: mCherry driven by EF1a promoter.
INSERT: DR-SgRNA driven by U6 promoter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-U6-DR-SgRNA-DR-EF1a-mCherry-SV40 was a gift from Hui Yang (Addgene plasmid # 154002 ; http://n2t.net/addgene:154002 ; RRID:Addgene_154002)

  • For your References section:

    Modulation of metabolic functions through Cas13d-mediated gene knockdown in liver. He B, Peng W, Huang J, Zhang H, Zhou Y, Yang X, Liu J, Li Z, Xu C, Xue M, Yang H, Huang P. Protein Cell. 2020 Mar 18. pii: 10.1007/s13238-020-00700-2. doi: 10.1007/s13238-020-00700-2. 10.1007/s13238-020-00700-2 PubMed 32185621
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