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Addgene

frh-1-B
(Plasmid #15400)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15400 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    L4440
  • Backbone size w/o insert (bp) 2676
  • Vector type
    RNAi

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Frataxin homologue
  • Alt name
    Frataxin
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    714
  • GenBank ID
    NC_003280
  • Entrez Gene
    frh-1 (a.k.a. CELE_F59G1.7)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (destroyed during cloning)
  • 3′ cloning site EcoRV (destroyed during cloning)
  • 5′ sequencing primer T7 Promoter
  • 3′ sequencing primer T7 Promoter
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    frh-1-B was a gift from Michael Ristow (Addgene plasmid # 15400 ; http://n2t.net/addgene:15400 ; RRID:Addgene_15400)

  • For your References section:

    Impaired respiration is positively correlated with decreased life span in Caenorhabditis elegans models of Friedreich Ataxia. Zarse K, Schulz TJ, Birringer M, Ristow M. FASEB J. 2007 Apr . 21(4):1271-5. 10.1096/fj.06-6994com PubMed 17215485
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