pDD2148
(Plasmid #153968)

• Purpose: pCMV6-Entry vector encoding human IRAK1 protein 196F
• Depositing Lab: Dirk Dittmer
• Publication: Seltzer et al J Virol. 2020 May 4;94(10). pii: JVI.02123-19. doi: 10.1128/JVI.02123-19. Print 2020 May 4.

Backbone

• Vector backbone: pCMV6-Entry
• Backbone size w/o insert (bp): 4500
• Total vector size (bp): 7500
• Modifications to backbone: IRAK1 position 196 was changed from S196F
• Vector type: Mammalian Expression
• Selectable markers: Gentamicin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: IRAK1
• Species: H. sapiens (human)
• Insert Size (bp): 2800
• GenBank ID: NM_001569
• Entrez Gene: IRAK1 (a.k.a. IRAK, pelle)
• Tag / Fusion Protein:
  • Myc-DDK

Resource Information

• A portion of this plasmid was derived from a plasmid made by: We purchased the original plasmid from Origene CAT#: RC221544 and had blue heron modify it so that IRAK1 is 196F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry