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Addgene

pMKO.1 puro-luc (shRNA)
(Plasmid #153962)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 153962 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMKO.1 puro (Addgene #8452)
  • Backbone manufacturer
    Robert Weinberg
  • Backbone size w/o insert (bp) 6340
  • Total vector size (bp) 6371
  • Modifications to backbone
    This plasmid was made by removing a shRNA against p53 from pMKO.1 puro p53 shRNA 2 (Addgene #10672, provided by William Hahn) and instead incorporating a shRNA against firefly luciferase (shVC)
  • Vector type
    Mammalian Expression, Retroviral, RNAi, Luciferase
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    shRNA against firefly luciferase (19-bp-long target)
  • Alt name
    shVC
  • gRNA/shRNA sequence
    CTTACGCTGAGTACTTCGA
  • Species
    Synthetic; from firefly
  • Promoter U6 promoter
  • Tag / Fusion Protein
    • N/A

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer -
  • 3′ sequencing primer -
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMKO.1 puro-luc (shRNA) was a gift from Hiroyuki Konishi (Addgene plasmid # 153962 ; http://n2t.net/addgene:153962 ; RRID:Addgene_153962)

  • For your References section:

    Tandem Paired Nicking Promotes Precise Genome Editing with Scarce Interference by p53. Hyodo T, Rahman ML, Karnan S, Ito T, Toyoda A, Ota A, Wahiduzzaman M, Tsuzuki S, Okada Y, Hosokawa Y, Konishi H. Cell Rep. 2020 Jan 28;30(4):1195-1207.e7. doi: 10.1016/j.celrep.2019.12.064. 10.1016/j.celrep.2019.12.064 PubMed 31995758
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