FLAG-mTIP49a
(Plasmid #15357)

• Depositing Labs: Joan Conaway, Ronald Conaway
• Publication: Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-12.

Backbone

• Vector backbone: pcDNA5 FRT
• Backbone size w/o insert (bp): 5070
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TATA binding protein interacting protein 49 kDa
• Alt name: TIP49a
• Species: M. musculus (mouse)
• Insert Size (bp): 1370
• Entrez Gene: Ruvbl1 (a.k.a. 2510009G06Rik, Pontin52, Tip49a)
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry